Unveiling the Therapeutic Potential of Methanolic Extract of Amaranthus dubius: Anticancer, Antibacterial and Antioxidant Activity

Abstract

Many phytochemicals, present in plant, are very important for healing from different diseases. The seeds of Amaranthus dubius, in particular has bioactive compounds which may have anti-cancer, anti-microbial and anti-oxidant functions. This study aimed to assess the anti-oxidant, anti-cancer activity of methanol extract of A. dubius leaf. We have isolated methanol extract from clean shed dried leaf of the A. dubius plant. We have used different concentrations of the extract and assessed the cell viability using MTT assay on L-132 lung cancer cell line, assessed the antibacterial activity through disc diffusion on human pathogenic bacteria, assessed antioxidant activity using DPPH scavenging action. Our results indicated that methanol extract of A. dubius plant leaf induced apoptosis of 70% L-132 cell at the concentration of 500 μg/ml. The extract showed best bacterial growth inhibition at 500mg/disc concentration against Shigella boydii. The extract also showed 66% DPPH scavenging activity where we have used ascorbic acid as positive control. In addition, endeavour to describe the antibacterial properties of the plant. Altogether, the methanol extract of the A. dubius plant leaf showed potential medicinal effects. In future, active compound(s) need to be isolated and introduce in animal model to see the best beneficial effect.