In-Vitro Effect of Most Potent Ethyl-Acetate Solvent Fraction of Curcuma Amada Rhizomes for the Management of Diabetes Induced Male Reproductive Impairments

Abstract

Curcuma amada is employed as a medicinal herb in traditional medicinal practices. The aim of this research was to determine if the rhizome of Curcuma amada could directly improve the male reproductive system of diabetic rats. To explore whether the ethylacetate fraction of Curcuma amada rhizomes has a direct antioxidative impact on STZinduced diabetic animals, the activities of antioxidant enzymes such as catalase and superoxide dismutase, as well as the level of Thiobarbituric acid reactive substances in testicular tissue, were evaluated. The activities of Δ5, 3β- hydroxysteroid dehydrogenase, and 17β- hydroxysteroid dehydrogenase were also evaluated to determine the direct impact of the ethyl-acetate fraction on the androgenic domain. When the testes of diabetic rats were directly exposed to the ethyl-acetate fraction of the plant, it did not lead to any significant recovery in the said enzyme activities. Following the direct exposure of the ethyl-acetate fraction of Curcuma amada rhizomes to the testes of type 1 diabetic rats, said parameters associated with oxidative stress, exhibited a positive improvement towards control. This improvement could be ascribed to the antioxidative phytomolecules present in the ethyl-acetate fraction of the plant, which effectively suppressed testicular oxidative stress by scavenging free radicals in the target tissue.