https://ir.vidyasagar.ac.in/jspui/handle/123456789/1314 Sun, 26 Apr 2026 07:49:19 GMT 2026-04-26T07:49:19Z https://ir.vidyasagar.ac.in/jspui/handle/123456789/6310 Title: Consequence of dietary n-Acetyl Cysteine on Arsenic mediated female reproductive hazards Authors: Dash, Moumita Abstract: Background: Arsenic contamination consistently provides a negative impact especially by drinking water and resulted in many fatal disorders. Numerous treatment approaches have been availed to combat arsenic assisted distress with plenty of side effects. Antioxidants with chelation properties become more advantageous to treat arsenication with the least toxicity. Aims & objectives: Present study highlighted the expansion of risk-free, safe, and nontoxic treatment strategy to oppose arsenication. The whole experimentation also focused on the antioxidant plus chelating possession and remedial index of NAC against sodium arsenite driven reproductive complications in rat model. Methods: In different mode of treatment sodium arsenite and NAC were employed wherein 10 mg/kg body weight of sodium arsenite and 100 mg/kg body weight of NAC were applied respectively. But the dietary application of NAC was provided at the dose of 250 mg/kg body weight. Arsenic mediated oxidative stress was justified by the assay of MDA-CD, SOD, catalase plus GPx activities along with NPSH. Arsenic interfered usual reproductive functions were justified by the assay of ∆ HSD and 17β-HSD, and also ovarian hormones like LH, estradiol, and FSH were studying the regulation of reproductive functions of female rats. Serum SGOT, SGPT, creatinine were assayed to indicate hepato-renal functionality. Few inflammatory indicators were assayed such as TNF-α, IL-6, NF-B and also apoptotic markers (Bax, Bcl-2, p53) were assessed. Serum level of vitamin B I, folic acid, homocysteine were assayed as well. DNA damage, histopathology of 12 5 , 3β- , MT-ovarian-uterine tissues, immunohistochemistry for affirmation of ER-α was also illustrated. Results: Arsenic aggravated oxidative stress implicated with higher levels of MDA- CD with lowering activity of antioxidative enzymes. Steroidogenic action in ovary was suppressed notably by diminishing the level of gonadotropins and estradiol in circulation. This causes structural dysintegration of ovarian-uterine tissues. Arsenic also promulgated DNA abnormalities by higher and significant discharge of inflammatory indicators and stimulating the pro-apoptotic gene manifestation. The controlling function of NAC worked against all these abnormal circumstances and brought back in homeostasis. Arsenic primed over-creation of oxidative stress was neutralized by NAC via improving antioxidant enzymatic activity in repro-organs. NAC guided improvement of gonadotropin’s utility significantly favoured ovarian steroidogenesis. The inflammatory condition following arsenication was well managed by the accessibility of NAC and also controlled the apoptotic progression. The important vitamin’s level was up-surged in NAC availed group while protected against homocysteine surge in arsenite challenged group. Conclusion: The above said outcomes specified that NAC may be a novel bio- component against arsenic and its proposed adverse implications. Antioxidant plus chelating conformity of NAC combat against arsenic directed oxidative stress. Also it has anti-apoptotic and anti-inflammatory possessions and thus maintains the system from arsenic aggravated complications. Wed, 06 Oct 2021 00:00:00 GMT https://ir.vidyasagar.ac.in/jspui/handle/123456789/6310 2021-10-06T00:00:00Z https://ir.vidyasagar.ac.in/jspui/handle/123456789/6240 Title: Impact of Vitamin B12 and Folic acid on Arsenic Induced Female Reproductive Status of Albino Rats Authors: Deb, Bimal Abstract: The present study elucidated the profound protective role of vitamin B and folic acid against arsenic-mediated reproductive toxicity in female rats. Ingestion of sodium-arsenite via drinking water [0.4 ppm/100 g body weight (b.wt.)/day] followed by the co-administration of vitamin B and folic acid (0.07 μg and 4.0 μg respectively/100 g b.wt./day) in Wistar rats represented a notable protection against arsenic-induced disruption of female gonadal function, ROS generation and DNA fragmentation of ovarian and uterine tissues as well as disorganization of uteroovarian histoarchitecture. However, arsenication impaired the action of the free radical scavenging enzymes superoxide dismutase (SOD) and catalase, peroxidase (POD) in ovarian and uterine tissue, with a concomitant elevation in lipid peroxidation in the reproductive organs. Arsenic ingestion resulted significant drop in the circulating follicle-stimulating hormone (FSH), leutinizing hormone (LH), and estradiol along with retarded activities of  12 5 ,3β -hydroxysteroid dehydrogenase (5,3β-HSD) and 17β-hydroxysteroid dehydrogenase (17β-HSD). Arsenicated rats showed irregular estrous cycle dominated by lengthy diestrus. Our previous work on arsenic-exposed humans was associated with DNA injury and carcinogenesis. Here, we demonstrated that the supplementation of aforesaid physiological/therapeutic dose of vitamin B and folate protected the rodents appreciably from arsenicinduced DNA damage (DNA fragmentation and comet assay) and ovarian and uterine tissue disintegration (histoarchitecture, HE staining). Vitamin supplementation mitigated the arsenic mediated reproductive injury by preventing abundant generation of free radicals. Restrained generation of free radicals may be correlated to the protection of DNA stability and reproductive organs morphology. This study highlighted that the decisive role of vitamin B 12 and folic acid in ameliorating arsenic-mediated reproductive disorder. Mon, 02 Aug 2021 00:00:00 GMT https://ir.vidyasagar.ac.in/jspui/handle/123456789/6240 2021-08-02T00:00:00Z https://ir.vidyasagar.ac.in/jspui/handle/123456789/6059 Title: Role of Arjunolic acid and vitamin B12 on Arsenic induced Ovarian Malfunction: An approach through the study of S-Adenosine Methionine Pool Authors: Maity, Moulima Abstract: People of wide area of India are slowly poisoned through the arsenic contaminated drinking water. The affected people of low economic groups from rural area are suffering with skin cancer, metabolic disorders, reproductive hazards, infertility etc. It is global challenge to prevent arsenic induced reproductive toxicity. There are very limited chelating agents found in the market (BAL, DMSA, DMPS) to prevent arsenic induced health hazards but these have noninvasive and painful treatment strategy. Hence, we have planned to develop an easily available cost-effective drug against arsenic toxicity which has no side effects. To fulfill this endeavor we have chosen arjunolic acid and vitamin B12 to observe the effects against arsenic induced female repro-toxicity. Sodium arsenite (1.0mg/100gm body weight) was given to the adult female Wistar strain rats with arjunolic acid (1.0mg/100gm body weight) and vitamin B12 (0.09μg/100gm body weight) alone or in combination to find out preventive, protective and curative effect of these two. Arjunolic acid and vitamin B12 could prevent arsenic induced ROS- production through the alteration of the antioxidants activities, ovarian steroidogenesis and inflammatory response in preventive, protective and curative manner as focused from in vivo experimental model. These mitigating effects may involve an indirect mechanism associated with a critical role of hypothalamico-pituitary-ovarian axis, although arjunolic acid and B12 had shown its ability to exert its effect directly on the reproductive organs as revealed from the experiment on in vitro model. Sun, 18 Apr 2021 00:00:00 GMT https://ir.vidyasagar.ac.in/jspui/handle/123456789/6059 2021-04-18T00:00:00Z https://ir.vidyasagar.ac.in/jspui/handle/123456789/5738 Title: Analysis of therapeutic strength of Curcumin, Chitosan and other Polysaccharides for the management of arsenic induced Ovarian and Uterine Anarchy Authors: Parveen, Hasina Abstract: Arsenic is a nonessential trace element. A large population worldwide is being affected by sodium arsenite polluted drinking water. Arsenic intoxication is responsible for the severe health hazards mainly infertility in humans and animals. Various treatment approaches: DMSA and BAL becoming a challenge to manage the arsenic-induced reproductive malfunction. Sometimes this chelation remedy for arsenic induced health hazards gives rise to moderate to severe side effects. Now a day’s researchers tried to use different natural herbal plant extracts and polymer-based nanoparticles to overcome the problem of arsenisation in animal model. Hence, the present thesis work emphasized to mature a non- invasive therapeutic model using chitosan, curcumin and pectic polysaccharide. The present study focused the structure of the extracted pectic polysaccharide (CCPS) of bitter gourd (Momordica charantia) contains D-methyl galacturonate and D-galactose in 4:1 molar ratios. FTIR study of CCPS indicates that it has hydroxyl groups to bind with arsenic in its structure. Series of negatively charged galacturonate remains in CCPS provides the better potential activity for the cation chelation. The synthesized water soluble encapsulated curcumin chitosan nanoparticles (ECNPs) having a diameter of 8-40 nanometres appeared to relief arsenic-mediated hazards. These studies fulfill to search out the efficacy of curcumin, CCPS and ECNPs against sodium-arsenite mediated female repro-toxicity. The solo or combined treatment mode at 20 mg/ Kg BW of the curcumin, 2.0 mg/ Kg BW of CCPS and 1.0 mg/ Kg BW of ECNPs doses were selected against 10 mg/ Kg BW of sodium arsenite. The curcumin, CCPS and ECNPs extensively attenuate the arsenic-mediated oxidative stress and lipid peroxidation level in uterus and ovary. Treatment of these biomolecules mitigates the suppression of ovarian steroidogenesis in the arsenicated Wistar rats by regulating the estradiol receptor along with the normal tissue histo-architecture. Oral administration of curcumin and CCPS also suppress the inflammatory markers TNF-α, IL-6, and NF-қB in the arsenicated rats. Up-regulation of Bax, caspase-3, PARP, PCNA and phospho p53 in arsenicated rats was followed by down regulation of Bcl 2 and AKT respectively. CCPS significantly reverts back these arsenic induced expressional changes. Dietary CCPS also makes sure the successful fertility rate with healthy pups in arsenic fed rats. This study helps to understand the underlying mechanism of curcumin, CCPS and ECNPs in attenuating arsenic mediated uterine and ovarian dysfunction involving regulation of SAM pool components, B12 , folate and homocysteine. Moreover, the encapsulated curcumin-chitosan nanoparticles at tiny establish greater efficacy than that of higher dose of curcumin alone against the arsenic induced female repro-toxicity. Sat, 23 Jan 2021 00:00:00 GMT https://ir.vidyasagar.ac.in/jspui/handle/123456789/5738 2021-01-23T00:00:00Z